By: Pål Mugaas Jensen, editor at Landbased AQ. Article originally published in Norwegian.
What happens to water quality in a RAS facility when you add nanobubbles? And are there additional effects on system efficiency and fish health? These were some of the questions technology company, Moleaer, wanted to answer when they took a scientific approach at Lødingen Fisk.
The testing conducted by Moleaer at Lødingen Fisk has resulted in a report titled, Effect of Oxygen Nanobubbles on Performance and Water Quality in Recirculating Aquaculture Systems (RAS). In this article, Federico Pasini, senior R&D scientist at Moleaer, explains the findings.
Pasini states that the goal of the study was to quantify how introducing oxygen nanobubbles can improve water quality, reduce energy and water consumption through enhanced oxygenation, improved particle removal, increased nitrification rates, and prevention of biofilm. The study was carried out in two stages to evaluate both the short- and long-term effects on RAS.
The first stage was conducted to characterize the effects of nanobubbles within 48 hours of starting up the nanobubble generator (NBG). The second stage of measurements took place after 50 days of continuous NBG operation, with much larger fish and significantly higher oxygen requirements, during a period when the facility was operating at 60% of full capacity.
"We also studied what happened and how the process responded to the presence of nanobubbles. Finally, we examined the impact on the fish, though we still have some questions in that area. Nevertheless, we identified some interesting points for future studies," he says.
"So what happened immediately after you started the nanobubble generator?"
"As expected, we were able to detect oxygen nanobubbles in the process. Measuring nanobubbles or nanoparticles is not entirely simple; there are many variables, and precision and a strict protocol are required. We usually measure nanoparticles before the experiment as a baseline, without nanobubbles – that is, before the generators are started. We then continued to collect samples after the nanobubble generators were started, and we measured a net increase in each step of the process, averaging 60 million per ml," he says.
"And we know from our experiments in the laboratory and other experiences in the field that this concentration is definitely sufficient to see an effect on water, biology and to some extent, biochemistry. We therefore know that in general, regardless of which water system we treat, we expect a reaction at that concentration," says Pasini.
He says that in about three hours, they observed a net increase of about 140 million nanobubbles per milliliter, with an average size of 170 nanometers. These nanobubbles disperse and dilute throughout the system, resulting in this average concentration in the process.
He explains that by creating such small and stable bubbles, we can ensure a very high oxygen release efficiency, which was immediately evident through the metrics at the four sampling points. "We had an immediate increase in oxygen at all stages of the process."
An interesting and clear effect was also observed in scrubbing and removing biofilm (see fact box below).
"We know nanobubbles will be involved in one way or another in everything that has to do with surface interactions. In this case, they cause some of the biofilm and some of the old coating on the surfaces of the tanks and pipes to loosen. Within 24 to 48 hours, we see an increase in solids, which then dissolve over time, resulting in a reduced need for ozone.
Nanobubbles therefore create a scouring effect, where certain types of biofilm detach from surfaces, enter the water, and can then be filtered out.
The nanobubbles themselves have a very hard surface, and with the speed at which they move, they are actually able to scour loose biological material, suspending it in the water stream. It can then go to the drum filters, where it is removed.
"This is why we see an increase in TSS (total suspended solids) at almost every step of the process, especially from the fish tank. We observed more solids during the 48-hour period, indicating the release of more particles. The feeding rate was the same, so it wasn't due to any other conditions in the tank.
"We also tested the oxygen release efficiency and we measured an efficiency of over 90% at that level of gas injection, which was low at the beginning of the process. We also saw indications of improved disinfection, which were later confirmed by the UV and ozone data," Pasini says.
"The most interesting thing, in my opinion, was the beneficial effect on the biofilter, especially on nitrification," he says (See Figure 2). "Because in the end, it is nitrification that determines how much load the plant can withstand."
"Even though you can add more oxygen, increase feeding, and increase fish density, you will still need a stronger and more efficient biofilter to handle all the ammonia, so you don't create any risk to fish welfare."
Figure 2: Nitrite effluent from biofilter
They observed that the presence of nanobubbles had an immediate positive effect on nitrification performance.
Biological nitrification is a process that involves specific types of bacteria, divided into two categories that perform two different processes. First, AOB bacteria (aerobic bacteria) that use oxygen to break down ammonia into NO₂ (nitrite). Then, another group of bacteria, called NOB (nitrite-oxidizing bacteria), takes this nitrite and converts it into NO₃ (nitrate), which is the end product of biological nitrification.
Nitrate is the least toxic form for fish, while nitrite is highly toxic even at low concentrations.
"It is therefore very important that we prevent the accumulation of nitrite, which is a symptom of a partial nitrification process. We show that with nanobubbles, we help to complete the step that was previously limiting, and overall we have a positive effect on water quality, which is very important since nitrite is so toxic to fish," he says.
The nanobubbles adhere to surfaces and create a gas coating on them.
"We can see this as a beneficial effect on turbidity. After 50 days, when the fish were much larger and they were given much more feed, we had a much clearer water than when we started," says Pasini.
Another valuable observation highlighted is that dissolved oxygen increased at each stage of the process.The water quality in the tanks was significantly improved after nanobubbles had been allowed to work for 50 days.
"And what is more relevant is that we increased the utilization of oxygen by 60 percent compared to constant oxygenation. We were able to save a lot of oxygen compared to what they would normally use in the plant. This is a very strong economic argument for this type of technology," he says.
Federico Pasini says they also confirmed that nitrification was improved with nanobubbles in the water (see also Figure 2).
"The most important source of ammonia in the process is the feed, and feeding increased throughout the experiment. But we saw that nitrite levels were getting lower and lower, which means that the performance of the biofilter had improved."
"We also saw that CO₂ levels followed roughly the same trend, so it may appear that nanobubbles also have a positive effect on degassing, probably as a result of a better carbon conversion in the biofilter. So overall, we improved the water quality and reduced oxygen consumption," he says.
The efficiency of the biofilter, measured as the nitrification rate, was found to increase by over 60%.
As a result of the cleaning and scouring effect and the reduced turbidity, the overall specific consumption of ozone per tonne of biomass also decreased by almost 70%.
"This confirmed that we had a positive effect on biofilm prevention, surface cleaning, disinfection, and the overall turbidity of the water," says Pasini.
The effects on water quality also led to some interesting observations, which he adds they will continue to investigate further.
"More studies will be required to really understand what is happening in terms of fish welfare. But in general, we saw indications of positive effects on fish welfare in terms of feed factor and relative growth index. Despite the observations we made and the data we collected, we absolutely need to investigate further before drawing any final conclusions," he emphasizes.
"What we can conclude from the studies is that we generally had higher stability in dissolved oxygen (DO), as the oxygen content was higher overall and less variable throughout the process. This can certainly have a positive effect on fish welfare.
"The fact that the turbidity in the fish tank was reduced can also have a positive effect on fish welfare, as well as prevent fouling and bacterial formation to a certain extent.
"The improved biofilter efficiency, generally lower concentration of ammonia and nitrite, and lower CO₂ concentration are all parameters that contribute to a healthier environment for the fish.
Pasini says when it comes to further research efforts related to fish health, they will collaborate with other research institutions that have the expertise to study the medical and metabolic aspects of fish growth in general.
"We will do our best to provide them with as much knowledge about nanobubbles as we can offer, to help solve this puzzle," he concludes.